Structural highlights
3os6 is a 4 chain structure with sequence from Bacillus anthracis (strain ames). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , |
| NonStd Res: | |
| Gene: | BAS2205, BA_2369, dhbC, GBAA2369, GBAA_2369 (Bacillus anthracis (strain Ames)) |
| Activity: | Isochorismate synthase, with EC number 5.4.4.2 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
The isochorismate synthase DhbC from Bacillus anthracis is essential for the biosynthesis of the siderophore bacillibactin by this pathogenic bacterium. The structure of the selenomethionine-substituted protein was determined to 2.4 A resolution using single-wavelength anomalous diffraction. B. anthracis DhbC bears the strongest resemblance to the Escherichia coli isochorismate synthase EntC, which is involved in the biosynthesis of another siderophore, namely enterobactin. Both proteins adopt the characteristic fold of other chorismate-utilizing enzymes, which are involved in the biosynthesis of various products, including siderophores, menaquinone and tryptophan. The conservation of the active-site residues, as well as their spatial arrangement, suggests that these enzymes share a common Mg(2+)-dependent catalytic mechanism.
Structure of isochorismate synthase DhbC from Bacillus anthracis.,Domagalski MJ, Tkaczuk KL, Chruszcz M, Skarina T, Onopriyenko O, Cymborowski M, Grabowski M, Savchenko A, Minor W Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):956-61. doi: , 10.1107/S1744309113021246. Epub 2013 Aug 19. PMID:23989140[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Domagalski MJ, Tkaczuk KL, Chruszcz M, Skarina T, Onopriyenko O, Cymborowski M, Grabowski M, Savchenko A, Minor W. Structure of isochorismate synthase DhbC from Bacillus anthracis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):956-61. doi: , 10.1107/S1744309113021246. Epub 2013 Aug 19. PMID:23989140 doi:10.1107/S1744309113021246
