BASIL2022GV3HDT

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Contents 1 Characterizing Putative Kinase 3HDT 2 Introduction 3 Online Resources (bioinformatic software) 3.1 BLASTp 3.2 Pfam 3.3 DALI 3.4 PANZ 4 Docking 5 Laboratory Experiments 5.1 Coupled kinase assay 5.2 SDS-PAGE 6 Conclusion/Future Experiments 7 References

Characterizing Putative Kinase 3HDT

This is a default text for your page BASIL2022GV3HDT. Click above on edit this page to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia ^[1] or to the article describing Jmol ^[2] to the rescue. This protein (3HDT) is a putative kinase with possibly cytidylate kinase function utilizing ATP and dCMP as ligands.

Introduction

Online Resources (bioinformatic software)

We used a variety of in silico databases with our protein, 3HDT to find similarities with other amino acid sequences, protein family matches, and structural comparisons to known proteins in the PDB. Below are the recorded results and information from each database. From this information, a hypothesized function was created for 3HDT and potential substrates were selected such as dCMP.

BLASTp

Pfam

DALI

PANZ

Docking

Laboratory Experiments

Coupled kinase assay

SDS-PAGE

Conclusion/Future Experiments

This is a sample scene created with SAT to by Group, and another to make of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes.

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References

1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644