1f5q
From Proteopedia
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CRYSTAL STRUCTURE OF MURINE GAMMA HERPESVIRUS CYCLIN COMPLEXED TO HUMAN CYCLIN DEPENDENT KINASE 2
Overview
Several gamma-herpesviruses encode proteins related to the mammalian, cyclins, regulatory subunits of cyclin-dependent kinases (cdks) essential, for cell cycle progression. We report a 2.5 A crystal structure of a, full-length oncogenic viral cyclin from gamma-herpesvirus 68 complexed, with cdk2. The viral cyclin binds cdk2 with an orientation different from, cyclin A and makes several novel interactions at the interface, yet it, activates cdk2 by triggering conformational changes similar to cyclin A., Sequences within the viral cyclin N-terminus lock part of the cdk2 T-loop, within the core of the complex. These sequences and others are conserved, amongst the viral and cellular D-type cyclins, suggesting that this, structure has wider implications for other cyclin-cdk complexes. The, observed resistance of this viral cyclin-cdk complex to inhibition by the, p27(KIP:) cdk inhibitor is explained by sequence and conformational, variation in the cyclin rendering the p27(KIP:)-binding site on the cyclin, subunit non-functional.
About this Structure
1F5Q is a Protein complex structure of sequences from Homo sapiens and Murid herpesvirus 1 with CL as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a gamma-herpesvirus cyclin-cdk complex., Card GL, Knowles P, Laman H, Jones N, McDonald NQ, EMBO J. 2000 Jun 15;19(12):2877-88. PMID:10856233
Page seeded by OCA on Mon Nov 12 16:49:31 2007
