The structure comprises 490 amino acids, a metal ion and a phosphate ion. Each protomer consisted of four do mains: core domain (CD), extra domain 1(ED1), extra domain 2 (ED2), and extra domain 3 (ED3).
CD had a canonical TIM-barrel or (βα)8-barrel scaffold, comprising an interior of eight par allel β-strands in a barrel structure, surrounded by eight exterior α helices (Fig. S2a). ED1 showed a Rossmann-like α/β/α sandwich fold with three α-helices and five β-strands; ED2, an α + β protein fold with four α-helices and two β-strands; ED3, an all-α protein fold with seven α-helices. ED1 and ED2 were parallel to the TIM-barrel structure on each side, while ED3 lay on top of CD, resembling a cap . A metal ion, at the top central position of the TIM-barrel structure, was bound with the metal-coordination loop in the interface between CD and ED2 and a phosphate ion was covalently bound to Ser345 in the interface between CD and ED3
(Fig. S2c).
The metal ion has key roles in numerous sugar metabolizing en zymes. In the apo ClUxaE structure, a divalent metal ion was bound with His160, Ser269, Asp272, Glu273, His342, and a water molecule in an octahedral geometry (Fig. 2a). In the ClUxaE-2Gly structure, the metal ion was shifted toward the first glycerol molecule (1Gly) in the substrate binding pocket by 4.3 Å and coordinated with Asp159, His160, Asp272, His342, and two hydroxyl groups from 1Gly (Fig. 2b–
1,6-bisphosphate aldolase (FBA) [27,28]. In the transition, Arg125 formed a hydrogen bond with the second glycerol molecule (2Gly) and Glu273 lost the metal coordination and formed a hydrogen bond with the conformation changed Arg125.
You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue.
Function
Epimerases and racemases are isomerase enzymes that catalyze the inversion of stereochemistry in biological molecules. Epimerases catalyze the stereochemical inversion of the configuration about an asymmetric carbon atom in a substrate having more than one center of asymmetry, thus interconverting epimers. One classical exemple of Epimerase is UDP-galactose_4-epimerase, which is used in the final step of galactose metabolism - catalyzing the reversible conversion of UDP-galactose to UDP-glucose.
Relevance
Pectin is dadsada
Xylan is adasdsada
Pectin na dXylan is easiy conversed in tagaturonate and Pectin is easiy conversed in Fructuronate. To trasform easileyhas impact on...
Structural highlights
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