| Structural highlights
Function
[WRI1_ARATH] May be involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways (By similarity). Transcriptional activator involved in the activation of a subset of sugar-responsive genes and the control of carbon flow from sucrose import to oil accumulation in developing seeds. Binds to the GCC-box pathogenesis-related promoter element. Promotes sugar uptake and seed oil accumulation by glycolysis. Required for embryo development, seed germination and, indirectly, for seedling establishment. Negative regulator of the ABA-mediated germination inhibition.[1] [2] [3] [4] [5] [6]
Publication Abstract from PubMed
Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, but the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved the structure of Arabidopsis WRI1 (AtWRI1) with its cognate double-stranded DNA (dsDNA), revealing two antiparallel beta sheets in the tandem AP2 domains that intercalate into the adjacent major grooves of dsDNA to determine the sequence recognition specificity. We showed that AtWRI1 represented a previously unidentified structural fold and DNA-binding mode. Mutations of the key residues interacting with DNA element affected its binding affinity and oil biosynthesis when these variants were transiently expressed in tobacco leaves. Seed oil content was enhanced in stable transgenic wri1-1 expressing an AtWRI1 variant (W74R). Together, our findings offer a structural basis explaining WRI1 recognition and binding of DNA and suggest an alternative strategy to increase oil yield in crops through WRI1 bioengineering.
Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis.,Qiao Z, Kong Q, Tee WT, Lim ARQ, Teo MX, Olieric V, Low PM, Yang Y, Qian G, Ma W, Gao YG Sci Adv. 2022 Aug 26;8(34):eabq1211. doi: 10.1126/sciadv.abq1211. Epub 2022 Aug, 24. PMID:36001661[7]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ruuska SA, Girke T, Benning C, Ohlrogge JB. Contrapuntal networks of gene expression during Arabidopsis seed filling. Plant Cell. 2002 Jun;14(6):1191-206. PMID:12084821
- ↑ Cernac A, Benning C. WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis. Plant J. 2004 Nov;40(4):575-85. doi: 10.1111/j.1365-313X.2004.02235.x. PMID:15500472 doi:http://dx.doi.org/10.1111/j.1365-313X.2004.02235.x
- ↑ Masaki T, Mitsui N, Tsukagoshi H, Nishii T, Morikami A, Nakamura K. ACTIVATOR of Spomin::LUC1/WRINKLED1 of Arabidopsis thaliana transactivates sugar-inducible promoters. Plant Cell Physiol. 2005 Apr;46(4):547-56. doi: 10.1093/pcp/pci072. Epub 2005 Mar, 7. PMID:15753106 doi:http://dx.doi.org/10.1093/pcp/pci072
- ↑ Baud S, Graham IA. A spatiotemporal analysis of enzymatic activities associated with carbon metabolism in wild-type and mutant embryos of Arabidopsis using in situ histochemistry. Plant J. 2006 Apr;46(1):155-69. doi: 10.1111/j.1365-313X.2006.02682.x. PMID:16553903 doi:http://dx.doi.org/10.1111/j.1365-313X.2006.02682.x
- ↑ Cernac A, Andre C, Hoffmann-Benning S, Benning C. WRI1 is required for seed germination and seedling establishment. Plant Physiol. 2006 Jun;141(2):745-57. doi: 10.1104/pp.106.079574. Epub 2006 Apr , 21. PMID:16632590 doi:http://dx.doi.org/10.1104/pp.106.079574
- ↑ Focks N, Benning C. wrinkled1: A novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism. Plant Physiol. 1998 Sep;118(1):91-101. doi: 10.1104/pp.118.1.91. PMID:9733529 doi:http://dx.doi.org/10.1104/pp.118.1.91
- ↑ Qiao Z, Kong Q, Tee WT, Lim ARQ, Teo MX, Olieric V, Low PM, Yang Y, Qian G, Ma W, Gao YG. Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis. Sci Adv. 2022 Aug 26;8(34):eabq1211. doi: 10.1126/sciadv.abq1211. Epub 2022 Aug, 24. PMID:36001661 doi:http://dx.doi.org/10.1126/sciadv.abq1211
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