7phm

Structural highlights

Function

[ENCAP_MYCTU] Shell component of a type 1 encapsulin nanocompartment in situ; its cargo protects against oxidative stress at low pH. In situ and in E.coli assembles into proteinaceous shells about 22 nm in diameter with 2.5 nm thick walls (PubMed:34751132, PubMed:24855650). Cargo proteins are targeted to the interior via their C-terminal extensions; empty intact shells can be isolated in E.coli in the absence of cargo protein. There are at least 4 possible cargo proteins, DyP (encoded in the same locus), FolB, BfrB and Rv1762c; DyP and Rv1762c have been identified in vivo (PubMed:24855650). Probably involved in protection against oxidative damage from the host immune response (Probable) (PubMed:34751132). A T-cell antigen found in bacterial culture cell filtrates, stimulates mouse immune response. Does not have detectable bacteriocin activity (PubMed:9596740).^{[1] [2] [3] [4]}

References

1. ↑ Contreras H, Joens MS, McMath LM, Le VP, Tullius MV, Kimmey JM, Bionghi N, Horwitz MA, Fitzpatrick JA, Goulding CW. Characterization of a Mycobacterium tuberculosis nanocompartment and its potential cargo proteins. J Biol Chem. 2014 Jun 27;289(26):18279-89. doi: 10.1074/jbc.M114.570119. Epub, 2014 May 22. PMID:24855650 doi:http://dx.doi.org/10.1074/jbc.M114.570119
2. ↑ Lien KA, Dinshaw K, Nichols RJ, Cassidy-Amstutz C, Knight M, Singh R, Eltis LD, Savage DF, Stanley SA. A nanocompartment system contributes to defense against oxidative stress in Mycobacterium tuberculosis. Elife. 2021 Nov 9;10. pii: 74358. doi: 10.7554/eLife.74358. PMID:34751132 doi:http://dx.doi.org/10.7554/eLife.74358
3. ↑ Rosenkrands I, Rasmussen PB, Carnio M, Jacobsen S, Theisen M, Andersen P. Identification and characterization of a 29-kilodalton protein from Mycobacterium tuberculosis culture filtrate recognized by mouse memory effector cells. Infect Immun. 1998 Jun;66(6):2728-35. doi: 10.1128/IAI.66.6.2728-2735.1998. PMID:9596740 doi:http://dx.doi.org/10.1128/IAI.66.6.2728-2735.1998
4. ↑ Contreras H, Joens MS, McMath LM, Le VP, Tullius MV, Kimmey JM, Bionghi N, Horwitz MA, Fitzpatrick JA, Goulding CW. Characterization of a Mycobacterium tuberculosis nanocompartment and its potential cargo proteins. J Biol Chem. 2014 Jun 27;289(26):18279-89. doi: 10.1074/jbc.M114.570119. Epub, 2014 May 22. PMID:24855650 doi:http://dx.doi.org/10.1074/jbc.M114.570119