1hae
From Proteopedia
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HEREGULIN-ALPHA EPIDERMAL GROWTH FACTOR-LIKE DOMAIN, NMR, 20 STRUCTURES
Contents |
Overview
The solution structure of the 63-residue heregulin-alpha (HRG-alpha), epidermal growth factor (EGF)-like domain, corresponding to residues, 177-239 of HRG-alpha, has been determined to high resolution using data, from two-dimensional and three-dimensional homo- and heteronuclear NMR, spectroscopy. The structure is based on a total of 887 internuclear, distance and dihedral restraints derived from data obtained using, unlabeled and uniformly 15N-labeled protein samples, at pH 4.5, 20 degrees, C. A total of 20 structures were calculated using a hybrid distance, geometry-simulated annealing approach with the program DGII, followed by, restrained molecular dynamics using the program DISCOVER. The average, maximum violations are 0.12 +/- 0.01 angstroms and 1.4 +/- 0.3 degrees for, distance and dihedral restraints, respectively. The backbone, (N,C(alpha),C) atomic rms distribution about the mean coordinates for, residues 3-23 and 31-49 is 0.29 +/- 0/07 angstroms. The N-and C-terminal, residues (1-2 and 50-63) and 24-30 are disordered. Comparison of the, HRG-alpha EGF-like domain structure with the previously determined, structure of human EGF [Hommel et al. (1992) J. Mol. Biol. 227, 271-282], reveals a high degree of structural similarity; excluding the N-terminal, region (residues 1-13), the disordered phi-loop region (residues 24-30), that contains a three-residue insertion in HRG-alpha relative to hEGF, and, the disordered C-terminal region (residues 50-63), the C(alpha) alignment, between the HRG-alpha and hEGF minimized mean structures has a rms, difference of approximately 1 angstrom. In HRG-alpha the N-terminal, residues 2-6 form a well-defined beta strand rather than being disordered, as found for hEGF. This structural difference correlates with functional, data which suggest that the N-terminal region of the HRG-alpha EGF-like, domain is responsible for the observed receptor specificity differences, between HRG-alpha and EGF.
Disease
Known diseases associated with this structure: Fibromatosis, gingival, 2 OMIM:[605544], Schizophrenia, susceptibility to OMIM:[142445]
About this Structure
1HAE is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
High-resolution solution structure of the EGF-like domain of heregulin-alpha., Jacobsen NE, Abadi N, Sliwkowski MX, Reilly D, Skelton NJ, Fairbrother WJ, Biochemistry. 1996 Mar 19;35(11):3402-17. PMID:8639490
Page seeded by OCA on Mon Nov 12 17:14:50 2007
