Structural highlights
Function
TAP1_RAT Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin (By similarity).
Publication Abstract from PubMed
The ATP-binding cassette (ABC) transporter associated with antigen processing (TAP) participates in immune surveillance by moving proteasomal products into the endoplasmic reticulum (ER) lumen for major histocompatibility complex class I loading and cell surface presentation to cytotoxic T cells. Here we delineate the mechanistic basis for antigen translocation. Notably, TAP works as a molecular diode, translocating peptide substrates against the gradient in a strict unidirectional way. We reveal the importance of the D-loop at the dimer interface of the two nucleotide-binding domains (NBDs) in coupling substrate translocation with ATP hydrolysis and defining transport vectoriality. Substitution of the conserved aspartate, which coordinates the ATP-binding site, decreases NBD dimerization affinity and turns the unidirectional primary active pump into a passive bidirectional nucleotide-gated facilitator. Thus, ATP hydrolysis is not required for translocation per se, but is essential for both active and unidirectional transport. Our data provide detailed mechanistic insight into how heterodimeric ABC exporters operate.
Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter.,Grossmann N, Vakkasoglu AS, Hulpke S, Abele R, Gaudet R, Tampe R Nat Commun. 2014 Nov 7;5:5419. doi: 10.1038/ncomms6419. PMID:25377891[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grossmann N, Vakkasoglu AS, Hulpke S, Abele R, Gaudet R, Tampe R. Mechanistic determinants of the directionality and energetics of active export by a heterodimeric ABC transporter. Nat Commun. 2014 Nov 7;5:5419. doi: 10.1038/ncomms6419. PMID:25377891 doi:http://dx.doi.org/10.1038/ncomms6419
