| Structural highlights
Function
T2N1_LENAE An E and P subtype restriction enzyme that recognizes the double-stranded unmethylated sequence 5'-GCCGGC-3' and cleaves after C-3.[1] [2] [3]
Publication Abstract from PubMed
NAE:I is transformed from DNA endonuclease to DNA topoisomerase and recombinase by a single amino acid substitution. The crystal structure of NAE:I was solved at 2.3 A resolution and shows that NAE:I is a dimeric molecule with two domains per monomer. Each domain contains one potential DNA recognition motif corresponding to either endonuclease or topoisomerase activity. The N-terminal domain core folds like the other type II restriction endonucleases as well as lambda-exonuclease and the DNA repair enzymes MutH and Vsr, implying a common evolutionary origin and catalytic mechanism. The C-terminal domain contains a catabolite activator protein (CAP) motif present in many DNA-binding proteins, including the type IA and type II topoisomerases. Thus, the NAE:I structure implies that DNA processing enzymes evolved from a few common ancestors. NAE:I may be an evolutionary bridge between endonuclease and DNA processing enzymes.
Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase.,Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Jo K, Topal MD. DNA topoisomerase and recombinase activities in Nae I restriction endonuclease. Science. 1995 Mar 24;267(5205):1817-20. doi: 10.1126/science.7892605. PMID:7892605 doi:http://dx.doi.org/10.1126/science.7892605
- ↑ Jo K, Topal MD. Effects on NaeI-DNA recognition of the leucine to lysine substitution that transforms restriction endonuclease NaeI to a topoisomerase: a model for restriction endonuclease evolution. Nucleic Acids Res. 1996 Nov 1;24(21):4171-5. doi: 10.1093/nar/24.21.4171. PMID:8932368 doi:http://dx.doi.org/10.1093/nar/24.21.4171
- ↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SKh, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Kruger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY. A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res. 2003 Apr 1;31(7):1805-12. PMID:12654995
- ↑ Huai Q, Colandene JD, Chen Y, Luo F, Zhao Y, Topal MD, Ke H. Crystal structure of NaeI-an evolutionary bridge between DNA endonuclease and topoisomerase. EMBO J. 2000 Jun 15;19(12):3110-8. PMID:10856254 doi:10.1093/emboj/19.12.3110
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