1i2m
From Proteopedia
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RAN-RCC1-SO4 COMPLEX
Contents |
Overview
RCC1 (regulator of chromosome condensation), a beta propeller, chromatin-bound protein, is the guanine nucleotide exchange factor (GEF), for the nuclear GTP binding protein Ran. We report here the 1.8 A crystal, structure of a Ran*RCC1 complex in the absence of nucleotide, an, intermediate in the multistep GEF reaction. In contrast to previous, structures, the phosphate binding region of the nucleotide binding site is, perturbed only marginally, possibly due to the presence of a polyvalent, anion in the P loop. Biochemical experiments show that a sulfate ion, stabilizes the Ran*RCC1 complex and inhibits dissociation by guanine, nucleotides. Based on the available structural and biochemical evidence, we present a unified scenario for the GEF mechanism where interaction of, the P loop lysine with an acidic residue is a crucial element for the, overall reaction.
Disease
Known diseases associated with this structure: Alveolar soft-part sarcoma OMIM:[606236], Osteolysis, familial expansile OMIM:[603499], Paget disease of bone OMIM:[603499]
About this Structure
1I2M is a Protein complex structure of sequences from Homo sapiens with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for guanine nucleotide exchange on Ran by the regulator of chromosome condensation (RCC1)., Renault L, Kuhlmann J, Henkel A, Wittinghofer A, Cell. 2001 Apr 20;105(2):245-55. PMID:11336674
Page seeded by OCA on Mon Nov 12 17:25:37 2007
