7v2y

Structural highlights

Function

HPR1_YEAST Component the THO subcomplex of the TREX complex, which operates in coupling transcription elongation to mRNA export. The THO complex is recruited to transcribed genes and moves along the gene with the elongating polymerase during transcription. THO is important for stabilizing nascent RNA in the RNA polymerase II elongation complex by preventing formation of DNA:RNA hybrids behind the elongating polymerase. It functions in cotranscriptional formation of an export-competent messenger ribonucleoprotein particle (mRNP) by facilitating the loading of ATP-dependent RNA helicase SUB2 and the mRNA export factor YRA1 along the nascent mRNA.^{[1] [2] [3] [4]}

References

1. ↑ Jimeno S, Rondon AG, Luna R, Aguilera A. The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J. 2002 Jul 1;21(13):3526-35. PMID:12093753 doi:http://dx.doi.org/10.1093/emboj/cdf335
2. ↑ Rondon AG, Jimeno S, Garcia-Rubio M, Aguilera A. Molecular evidence that the eukaryotic THO/TREX complex is required for efficient transcription elongation. J Biol Chem. 2003 Oct 3;278(40):39037-43. Epub 2003 Jul 18. PMID:12871933 doi:http://dx.doi.org/10.1074/jbc.M305718200
3. ↑ Huertas P, Aguilera A. Cotranscriptionally formed DNA:RNA hybrids mediate transcription elongation impairment and transcription-associated recombination. Mol Cell. 2003 Sep;12(3):711-21. PMID:14527416
4. ↑ Abruzzi KC, Lacadie S, Rosbash M. Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes. EMBO J. 2004 Jul 7;23(13):2620-31. Epub 2004 Jun 10. PMID:15192704 doi:http://dx.doi.org/10.1038/sj.emboj.7600261