| Structural highlights
Function
MAPZ_STRR6 Early cell division protein that marks the future cell division site and supports proper FtsZ ring positioning.[HAMAP-Rule:MF_01941][1] [2]
Publication Abstract from PubMed
Accurate placement of the bacterial division site is a prerequisite for the generation of two viable and identical daughter cells. In Streptococcus pneumoniae, the positive regulatory mechanism involving the membrane protein MapZ positions precisely the conserved cell division protein FtsZ at the cell centre. Here we characterize the structure of the extracellular domain of MapZ and show that it displays a bi-modular structure composed of two subdomains separated by a flexible serine-rich linker. We further demonstrate in vivo that the N-terminal subdomain serves as a pedestal for the C-terminal subdomain, which determines the ability of MapZ to mark the division site. The C-terminal subdomain displays a patch of conserved amino acids and we show that this patch defines a structural motif crucial for MapZ function. Altogether, this structure-function analysis of MapZ provides the first molecular characterization of a positive regulatory process of bacterial cell division.
Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ.,Manuse S, Jean NL, Guinot M, Lavergne JP, Laguri C, Bougault CM, VanNieuwenhze MS, Grangeasse C, Simorre JP Nat Commun. 2016 Jun 27;7:12071. doi: 10.1038/ncomms12071. PMID:27346279[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Fleurie A, Lesterlin C, Manuse S, Zhao C, Cluzel C, Lavergne JP, Franz-Wachtel M, Macek B, Combet C, Kuru E, VanNieuwenhze MS, Brun YV, Sherratt D, Grangeasse C. MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Nature. 2014 Dec 11;516(7530):259-62. doi: 10.1038/nature13966. Epub 2014 Nov 26. PMID:25470041 doi:http://dx.doi.org/10.1038/nature13966
- ↑ Holeckova N, Doubravova L, Massidda O, Molle V, Buriankova K, Benada O, Kofronova O, Ulrych A, Branny P. LocZ is a new cell division protein involved in proper septum placement in Streptococcus pneumoniae. MBio. 2014 Dec 30;6(1):e01700-14. doi: 10.1128/mBio.01700-14. PMID:25550321 doi:http://dx.doi.org/10.1128/mBio.01700-14
- ↑ Manuse S, Jean NL, Guinot M, Lavergne JP, Laguri C, Bougault CM, VanNieuwenhze MS, Grangeasse C, Simorre JP. Structure-function analysis of the extracellular domain of the pneumococcal cell division site positioning protein MapZ. Nat Commun. 2016 Jun 27;7:12071. doi: 10.1038/ncomms12071. PMID:27346279 doi:http://dx.doi.org/10.1038/ncomms12071
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