1jmc
From Proteopedia
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SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420
Overview
The single-stranded-DNA-binding proteins (SSBs) are essential for DNA, function in prokaryotic and eukaryotic cells, mitochondria, phages and, viruses. The structures of four SSBs have been solved, but the molecular, details of the interaction of SSBs with DNA remain speculative. We report, here the crystal structure at 2.4 A resolution of the, single-stranded-DNA-binding domain of human replication protein A (RPA), bound to DNA. Replication protein A is a heterotrimeric SSB that is highly, conserved in eukaryotes. The largest subunit, RPA70, binds to, single-stranded (ss)DNA and mediates interactions with many cellular and, viral proteins. The DNA-binding domain, which lies in the middle of RPA70, comprises two structurally homologous subdomains oriented in tandem. The, ssDNA lies in a channel that extends from one subdomain to the other. The, structure of each RPA70 subdomain is similar to those of the bacteriophage, SSBs, indicating that the mechanism of ssDNA-binding is conserved.
About this Structure
1JMC is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA., Bochkarev A, Pfuetzner RA, Edwards AM, Frappier L, Nature. 1997 Jan 9;385(6612):176-81. PMID:8990123
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