1jo6
From Proteopedia
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Solution structure of the cytoplasmic N-terminus of the BK beta-subunit KCNMB2
Overview
The auxiliary beta-subunit KCNMB2 (beta(2)) endows the non-inactivating, large conductance Ca(2+)- and voltage-dependent potassium (BK) channel, with fast inactivation. This process is mediated by the N terminus of, KCNMB2 and closely resembles the "ball-and-chain"-type inactivation, observed in voltage-gated potassium channels. Here we investigated the, solution structure and function of the KCNMB2 N terminus (amino acids, 1-45, BKbeta(2)N) using NMR spectroscopy and patch clamp recordings., BKbeta(2)N completely inactivated BK channels when applied to the, cytoplasmic side; its interaction with the BK alpha-subunit is, characterized by a particularly slow dissociation rate and an affinity in, the upper nanomolar range. The BKbeta(2)N structure comprises two domains, connected by a flexible linker: the pore-blocking "ball domain" (formed by, residues 1-17) and the "chain domain" (between residues 20-45) linking it, to the membrane segment of KCNMB2. The ball domain is made up of a, flexible N terminus anchored at a well ordered loop-helix motif. The chain, domain consists of a 4-turn helix with an unfolded linker at its C, terminus. These structural properties explain the functional, characteristics of BKbeta(2)N-mediated inactivation.
About this Structure
1JO6 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure of the "ball-and-chain" domain of KCNMB2, the beta 2-subunit of large conductance Ca2+- and voltage-activated potassium channels., Bentrop D, Beyermann M, Wissmann R, Fakler B, J Biol Chem. 2001 Nov 9;276(45):42116-21. Epub 2001 Aug 21. PMID:11517232
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