Structural highlights
Function
EGR1_HUMAN Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
Publication Abstract from PubMed
The inducible transcription factor Egr-1 binds specifically to 9-bp target sequences containing two CpG sites that can potentially be methylated at four cytosine bases. Although it appears that complete CpG methylation would make an unfavorable steric clash in the previous crystal structures of the complexes with unmethylated or partially methylated DNA, our affinity data suggest that DNA recognition by Egr-1 is insensitive to CpG methylation. We have determined, at a 1.4-A resolution, the crystal structure of the Egr-1 zinc-finger complex with completely methylated target DNA. Structural comparison of the three different methylation states reveals why Egr-1 can recognize the target sequences regardless of CpG methylation.
Structural impact of complete CpG methylation within target DNA on specific complex formation of the inducible transcription factor Egr-1.,Zandarashvili L, White MA, Esadze A, Iwahara J FEBS Lett. 2015 May 19. pii: S0014-5793(15)00398-1. doi:, 10.1016/j.febslet.2015.05.022. PMID:25999311[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zandarashvili L, White MA, Esadze A, Iwahara J. Structural impact of complete CpG methylation within target DNA on specific complex formation of the inducible transcription factor Egr-1. FEBS Lett. 2015 May 19. pii: S0014-5793(15)00398-1. doi:, 10.1016/j.febslet.2015.05.022. PMID:25999311 doi:http://dx.doi.org/10.1016/j.febslet.2015.05.022
