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Publication Abstract from PubMed

Red blood cell antigens play critical roles in blood transfusion since donor incompatibilities can be lethal. Recipients with the rare total deficiency in H antigen, the O(h) Bombay phenotype, can only be transfused with group O(h) blood to avoid serious transfusion reactions. We discover FucOB from the mucin-degrading bacteria Akkermansia muciniphila as an alpha-1,2-fucosidase able to hydrolyze Type I, Type II, Type III and Type V H antigens to obtain the afucosylated Bombay phenotype in vitro. X-ray crystal structures of FucOB show a three-domain architecture, including a GH95 glycoside hydrolase. The structural data together with site-directed mutagenesis, enzymatic activity and computational methods provide molecular insights into substrate specificity and catalysis. Furthermore, using agglutination tests and flow cytometry-based techniques, we demonstrate the ability of FucOB to convert universal O type into rare Bombay type blood, providing exciting possibilities to facilitate transfusion in recipients/patients with Bombay phenotype.

Turning universal O into rare Bombay type blood.,Anso I, Naegeli A, Cifuente JO, Orrantia A, Andersson E, Zenarruzabeitia O, Moraleda-Montoya A, Garcia-Alija M, Corzana F, Del Orbe RA, Borrego F, Trastoy B, Sjogren J, Guerin ME Nat Commun. 2023 Mar 30;14(1):1765. doi: 10.1038/s41467-023-37324-z. PMID:36997505^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.