1juo

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spinqualitylabelsall models 1juo, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of Calcium-free Human Sorcin: A Member of the Penta-EF-Hand Protein Family

Overview

Sorcin is a 22 kD calcium-binding protein that is found in a wide variety, of cell types, such as heart, muscle, brain and adrenal medulla. It, belongs to the penta-EF-hand (PEF) protein family, which contains five, EF-hand motifs that associate with membranes in a calcium-dependent, manner. Prototypic members of this family are the calcium-binding domains, of calpain, such as calpain dVI. Full-length human sorcin has been, crystallized in the absence of calcium and the structure determined at 2.2, A resolution. Apart from an extended N-terminal portion, the sorcin, molecule has a globular shape. The C-terminal domain is predominantly, alpha-helical, containing eight alpha-helices and connecting loops, incorporating five EF hands. Sorcin forms dimers through the association, of the unpaired EF5, confirming this as the mode of association in the, dimerization of PEF proteins. Comparison with calpain dVI reveals that the, general folds of the individual EF-hand motifs are conserved, especially, that of EF1, the novel EF-hand motif characteristic of the family., Detailed structural comparisons of sorcin with other members of PEF, indicate that the EF-hand pair EF1-EF2 is likely to correspond to the two, physiologically relevant calcium-binding sites and that the, calcium-induced conformational change may be modest and localized within, this pair of EF-hands. Overall, the results derived from the structural, observations support the view that, in sorcin, calcium signaling takes, place through the first pair of EF-hands.

About this Structure

1JUO is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of calcium-free human sorcin: a member of the penta-EF-hand protein family., Xie X, Dwyer MD, Swenson L, Parker MH, Botfield MC, Protein Sci. 2001 Dec;10(12):2419-25. PMID:11714909

Page seeded by OCA on Mon Nov 12 17:44:36 2007