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1jxs

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1jxs

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Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor

Overview

Interleukin enhancer binding factor (ILF) binds to the interleukin-2, (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D, structure of the DNA-binding domain of ILF was determined by, multidimensional NMR spectroscopy. NMR structure analysis revealed that, the DNA-binding domain of ILF is a new member of the winged helix/forkhead, family, and that its wing 2 contains an extra alpha-helix. This is the, first study to report the presence of a C-terminal alpha-helix in place of, a typical wing 2 in a member of this family. This structural difference, may be responsible for the different DNA-binding specificity of ILF, compared to other winged helix/forkhead proteins. Our deletion studies of, the fragments of ILF also suggest that the C-terminal region plays a, regulatory role in DNA binding.

About this Structure

1JXS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a)., Liu PP, Chen YC, Li C, Hsieh YH, Chen SW, Chen SH, Jeng WY, Chuang WJ, Proteins. 2002 Dec 1;49(4):543-53. PMID:12402362

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