Structural highlights
Function
TUBR_CBCP A DNA-binding protein that is part of the type III partition system presumably used to ensure correct segregation of this bacteriophage. Binds to tubC (centromere-like site) DNA upstream of its own gene in a sequence-specific fashion (consensus TTGAC); binds to multiple sites in the tubC region, probably spreading from an initial site (PubMed:22538818, PubMed:29762781). Upon binding to tubC forms flexible loops over about 1 kb of DNA that forms 2 rings, covering tubC and the promoter region. This probably shuts off transcription of the operon. DNA is both bent and untwisted by TubR (PubMed:29762781). The TubR-tubC DNA complex binds to TubZ forming large bundles and decreasing TubZ's GTPase activity (PubMed:22538818).[1] [2]
References
- ↑ Oliva MA, Martin-Galiano AJ, Sakaguchi Y, Andreu JM. Tubulin homolog TubZ in a phage-encoded partition system. Proc Natl Acad Sci U S A. 2012 Apr 26. PMID:22538818 doi:10.1073/pnas.1121546109
- ↑ Martín-García B, Martín-González A, Carrasco C, Hernández-Arriaga AM, Ruíz-Quero R, Díaz-Orejas R, Aicart-Ramos C, Moreno-Herrero F, Oliva MA. The TubR-centromere complex adopts a double-ring segrosome structure in Type III partition systems. Nucleic Acids Res. 2018 Jun 20;46(11):5704-5716. PMID:29762781 doi:10.1093/nar/gky370
