Structural highlights
Publication Abstract from PubMed
The X-ray crystal structure of a brominated RNA helix with four mismatched base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to 2.1 A using the methods of multiwavelength anomalous diffraction (MAD) applied to the bromine K-absorption edge. There are three molecules in the asymmetric unit with unique crystal-packing environments, revealing true conformational variability at high resolution for this sequence. The structure shows that the sequence itself does not define a consistent pattern of solvent molecules, with the exception of the mismatched base pairs, implying that specific RNA-protein interactions would occur only with the nucleotides. There are a number of significant tertiary interactions, some of which are a result of the brominated base pairs and others that are directly mediated by the RNA 2' hydroxyl groups. The mismatched base pairs exhibit a solvent network as well as a stacking pattern with their nearest neighbors that validate previous thermodynamic analysis.
Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability.,Anderson AC, O'Neil RH, Filman DJ, Frederick CA Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Anderson AC, O'Neil RH, Filman DJ, Frederick CA. Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability. Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226
