Structural highlights
Function
CAPSD_BPHK7 Assembles to form an icosahedral capsid of 66 nm, with a T=7 laevo symmetry (PubMed:11000116, PubMed:21276801). Responsible for its self-assembly into a procapsid. The phage does not need to encode a separate scaffolfing protein because its capsid protein contains the delta domain that carries that function.[1] [2] [3] [4]
Publication Abstract from PubMed
Tailed bacteriophages and herpesviruses use a transient scaffold to assemble icosahedral capsids with hexameric capsomers on the faces and pentameric capsomers at all but one vertex where a 12-fold portal is thought to nucleate the assembly. How does the scaffold orchestrate this step? We have determined the portal vertex structure of the bacteriophage HK97 procapsid, where the scaffold is a domain of the major capsid protein. The scaffold forms rigid helix-turn-strand structures on the interior surfaces of all capsomers and is further stabilized around the portal, forming trimeric coiled-coil towers, two per surrounding capsomer. These 10 towers bind identically to 10 of 12 portal subunits, adopting a pseudo-12-fold organization that explains how the symmetry mismatch is managed at this early step.
A symmetry mismatch unraveled: How phage HK97 scaffold flexibly accommodates a 12-fold pore at a 5-fold viral capsid vertex.,Huet A, Oh B, Maurer J, Duda RL, Conway JF Sci Adv. 2023 Jun 16;9(24):eadg8868. doi: 10.1126/sciadv.adg8868. Epub 2023 Jun , 16. PMID:37327331[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wikoff WR, Liljas L, Duda RL, Tsuruta H, Hendrix RW, Johnson JE. Topologically linked protein rings in the bacteriophage HK97 capsid. Science. 2000 Sep 22;289(5487):2129-33. PMID:11000116
- ↑ Huang RK, Khayat R, Lee KK, Gertsman I, Duda RL, Hendrix RW, Johnson JE. The Prohead-I Structure of Bacteriophage HK97: Implications for Scaffold-Mediated Control of Particle Assembly and Maturation. J Mol Biol. 2011 Jan 27. PMID:21276801 doi:10.1016/j.jmb.2011.01.016
- ↑ Duda RL, Martincic K, Xie Z, Hendrix RW. Bacteriophage HK97 head assembly. FEMS Microbiol Rev. 1995 Aug;17(1-2):41-6. PMID:7669350 doi:10.1111/j.1574-6976.1995.tb00186.x
- ↑ Duda RL, Martincic K, Hendrix RW. Genetic basis of bacteriophage HK97 prohead assembly. J Mol Biol. 1995 Apr 7;247(4):636-47. PMID:7723020 doi:10.1006/jmbi.1994.0169
- ↑ Huet A, Oh B, Maurer J, Duda RL, Conway JF. A symmetry mismatch unraveled: How phage HK97 scaffold flexibly accommodates a 12-fold pore at a 5-fold viral capsid vertex. Sci Adv. 2023 Jun 16;9(24):eadg8868. PMID:37327331 doi:10.1126/sciadv.adg8868
