8t2i
From Proteopedia
Negative stain EM assembly of MYC, JAZ, and NINJA complex
Structural highlights
FunctionMYC3_ARATH Transcription factor involved in tryptophan, jasmonic acid (JA) and other stress-responsive gene regulation. With MYC2 and MYC4, controls additively subsets of JA-dependent responses. Can form complexes with all known glucosinolate-related MYBs to regulate glucosinolate biosynthesis. Binds to the G-box (5'-CACGTG-3') of promoters. Activates multiple TIFY/JAZ promoters.[1] [2] [3] [4] [5] Publication Abstract from PubMedJasmonates (JAs) are plant hormones with crucial roles in development and stress resilience. They activate MYC transcription factors by mediating the proteolysis of MYC inhibitors called JAZ. In the absence of JA, JAZ proteins bind and inhibit MYC through the assembly of MYC-JAZ-NINJA-TPL repressor complexes. However, JAZ and NINJA are predicted to be largely intrinsically unstructured, which precluded their experimental structure determination. Through a combination of biochemical, mutational, and biophysical analyses and AlphaFold-derived ColabFold modeling, we characterized JAZ-JAZ and JAZ-NINJA interactions and generated models with detailed, high confidence domain interfaces. We demonstrate that JAZ, NINJA, and MYC interface domains are dynamic in isolation and become stabilized in a stepwise order upon complex assembly. In contrast, most JAZ and NINJA regions outside of the interfaces remain highly dynamic and cannot be modeled in a single conformation. Our data indicate that the small JAZ ZIM motif mediates JAZ-JAZ and JAZ-NINJA interactions through separate surfaces, and further suggest that NINJA modulates JAZ dimerization. This study advances knowledge of JA signaling by providing insight into dynamics, interactions, and structure of the JAZ-NINJA core of the JA repressor complex. Assembly of JAZ-JAZ and JAZ-NINJA complexes in jasmonate signaling.,Zhou XE, Zhang Y, Yao J, Zheng J, Zhou Y, He Q, Moreno J, Lam VQ, Cao X, Sugimoto K, Vanegas-Cano L, Kariapper L, Suino-Powell K, Zhu Y, Novick S, Griffin PR, Zhang F, Howe GA, Melcher K Plant Commun. 2023 Jun 14:100639. doi: 10.1016/j.xplc.2023.100639. PMID:37322867[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Arabidopsis thaliana | Escherichia coli | Large Structures | Cao X | He Q | Kariapper L | Karsten M | Suino-Powell K | Zhang F | Zhang Y | Zhou XE | Zhou Y | Zhu Y
