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From Proteopedia
THE STRUCTURE OF D(TPGPCPGPCPA)2 AT 293K: COMPARISON OF THE EFFECT OF SEQUENCE AND TEMPERATURE
Overview
The crystal structure of a hexameric DNA fragment with the sequence d(TGCGCA)(2) has been solved and refined at 293 K at a resolution of 1.64 A. The molecule adopts a left-handed Z-type helical conformation which is common in alternating pyrimidine-purine sequences. The presence of A.T base pairs at the two terminals does not perturb the structure to any great degree. However, several sequence-specific microstructural changes are noticeable. The structure of the identical sequence determined at 120 K involving somewhat different crystallization conditions has been reported previously [Harper et al. (1998), Acta Cryst. D54, 1273-1284]. A comparison of the present structure with that at low temperature and with that of d(CGCGCG)(2) shows that the effect of the change in sequence is greater than the combined effect of changes in temperature and environment.
About this Structure
Full crystallographic information is available from OCA.
Reference
Structure of d(TGCGCA)2 at 293 K: comparison of the effects of sequence and temperature., Thiyagarajan S, Satheesh Kumar P, Rajan SS, Gautham N, Acta Crystallogr D Biol Crystallogr. 2002 Aug;58(Pt 8):1381-4. Epub 2002, Jul 20. PMID:12136163 Page seeded by OCA on Fri May 2 23:59:26 2008
