1mlx
From Proteopedia
Crystal Structure Analysis of a 2'-O-[2-(Methylthio)-ethyl]-Modified Oligodeoxynucleotide Duplex
Overview
A novel 2'-modification, 2'-O-[2-(methylthio)ethyl] or 2'-O-MTE, has been incorporated into oligonucleotides and evaluated for properties relevant to antisense activity. The results were compared with the previously characterized 2'-O-[2-(methoxy)ethyl] 2'-O-MOE modification. As expected, the 2'-O-MTE modified oligonucleotides exhibited improved binding to human serum albumin compared to the 2'-O-MOE modified oligonucleotides. The 2'-O-MTE oligonucleotides maintained high binding affinity to target RNA. Nuclease digestion of 2'-O-MTE oligonucleotides showed that they have limited resistance to exonuclease degradation. We analyzed the crystal structure of a decamer DNA duplex containing the 2'-O-MTE modifcation. Analysis of the crystal structure provides insight into the improved RNA binding affinity, protein binding affinity and limited resistance of 2'-O-MTE modified oligonucleotides to exonuclease degradation.
About this Structure
Full crystallographic information is available from OCA.
Reference
2'-O-[2-(methylthio)ethyl]-modified oligonucleotide: an analogue of 2'-O-[2-(methoxy)-ethyl]-modified oligonucleotide with improved protein binding properties and high binding affinity to target RNA., Prakash TP, Manoharan M, Kawasaki AM, Fraser AS, Lesnik EA, Sioufi N, Leeds JM, Teplova M, Egli M, Biochemistry. 2002 Oct 1;41(39):11642-8. PMID:12269806 Page seeded by OCA on Sat May 3 01:23:51 2008
Categories: Egli, M. | Fraser, A S. | Kawasaki, A M. | Leeds, J M. | Lesnik, E A. | Manoharan, M. | Prakash, T P. | Sioufi, N. | Teplova, M. | A-form | Antisense modification | Dna | Double helix | High resolution structure | Hydration | Nuclease resistance | Nucleic acid analogue | Protein binding affinity | Rna affinity
