Structural highlights
Function
RL27_ECOLI
Publication Abstract from PubMed
Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
Structural basis for mRNA and tRNA positioning on the ribosome.,Berk V, Zhang W, Pai RD, Cate JH Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:17038497[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Berk V, Zhang W, Pai RD, Cate JH. Structural basis for mRNA and tRNA positioning on the ribosome. Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:17038497
