5v00

Publication Abstract from PubMed

Cupins form one of the most functionally diverse superfamilies of proteins, with members performing a wide range of catalytic, non-catalytic, and regulatory functions. HutD is a predicted bicupin protein that is involved in histidine utilization (Hut) in Pseudomonas species. Previous genetic analyses have suggested that it limits the upper level of Hut pathway expression, but its mechanism of action is unknown. Here, we have determined the structure of PfluHutD at 1.74 A resolution in several crystallization conditions, and identified N-formyl-L-glutamate (a Hut pathway intermediate) is a potential ligand in vivo. This article is protected by copyright. All rights reserved.

Crystal structure of a bicupin protein HutD involved in histidine utilization in Pseudomonas.,Gerth ML, Liu Y, Jiao W, Zhang XX, Baker EN, Lott JS, Rainey PB, Johnston JM Proteins. 2017 Apr 6. doi: 10.1002/prot.25303. PMID:28383128^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.