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Publication Abstract from PubMed

Ivermectin acts as a positive allosteric modulator of several Cys-loop receptors including the glutamate-gated chloride channels (GluCls), gamma-aminobutyric acid receptors (GABAARs), glycine receptors (GlyRs), and neuronal alpha7-nicotinic receptors (alpha7 nAChRs). The crystal structure of Caenorhabditis elegans GluCl complexed with ivermectin revealed the details of its ivermectin binding site. Although the electron microscopy structure of zebrafish GlyRalpha1 complexed with ivermectin demonstrated a similar binding orientation, detailed structural information on the ivermectin binding and pore opening for Cys-loop receptors in vertebrates has been elusive. Here we present the crystal structures of human GlyRalpha3 in complex with ivermectin at 2.85 and 3.08 A resolution. Our structures allow us to explore in detail the molecular recognition of ivermectin by GlyRs, GABAARs, and alpha7 nAChRs. Comparisons with previous structures reveal how the ivermectin binding expands the ion channel pore. Our results hold promise in structure-based design of GlyR modulators for the treatment of neuropathic pain.

Crystal Structures of Human GlyRalpha3 Bound to Ivermectin.,Huang X, Chen H, Shaffer PL Structure. 2017 May 4. pii: S0969-2126(17)30108-9. doi:, 10.1016/j.str.2017.04.007. PMID:28479061^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.