Structural highlights
Function
LHX4_MOUSE May play a critical role in the development of respiratory control mechanisms and in the normal growth and maturation of the lung.ISL2_MOUSE Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways.
Publication Abstract from PubMed
Tandem beta zippers are modular complexes formed between repeated linear motifs and tandemly arrayed domains of partner proteins in which beta-strands form upon binding. Studies of such complexes, formed by LIM domain proteins and linear motifs in their intrinsically disordered partners, revealed spacer regions between the linear motifs that are relatively flexible but may affect the overall orientation of the binding modules. We demonstrate that mutation of a solvent exposed side chain in the spacer region of an LHX4-ISL2 complex has no significant effect on the structure of the complex, but decreases binding affinity, apparently by increasing flexibility of the linker.
Mutation in a flexible linker modulates binding affinity for modular complexes.,Stokes PH, Robertson NO, Silva APG, Estephan T, Trewhella J, Guss JM, Matthews JM Proteins. 2019 May;87(5):425-429. doi: 10.1002/prot.25675. Epub 2019 Mar 9. PMID:30788856[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Stokes PH, Robertson NO, Silva APG, Estephan T, Trewhella J, Guss JM, Matthews JM. Mutation in a flexible linker modulates binding affinity for modular complexes. Proteins. 2019 May;87(5):425-429. doi: 10.1002/prot.25675. Epub 2019 Mar 9. PMID:30788856 doi:http://dx.doi.org/10.1002/prot.25675
