Structural highlights
Publication Abstract from PubMed
DNA is an ideal molecule for the construction of 3D crystals with tunable properties owing to its high programmability based on canonical Watson-Crick base pairing, with crystal assembly in all three dimensions facilitated by immobile Holliday junctions and sticky end cohesion. Despite the promise of these systems, only a handful of unique crystal scaffolds have been reported. Herein, we describe a new crystal system with a repeating sequence that mediates the assembly of a 3D scaffold via a series of Holliday junctions linked together with complementary sticky ends. By using an optimized junction sequence, we could determine a high-resolution (2.7 A) structure containing R3 crystal symmetry, with a slight subsequent improvement (2.6 A) using a modified sticky-end sequence. The immobile Holliday junction sequence allowed us to produce crystals that provided unprecedented atomic detail. In addition, we expanded the crystal cavities by 50 % by adding an additional helical turn between junctions, and we solved the structure to 4.5 A resolution by molecular replacement.
A Self-Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High-Resolution Structural Detail.,Simmons CR, MacCulloch T, Zhang F, Liu Y, Stephanopoulos N, Yan H Angew Chem Int Ed Engl. 2020 Oct 12;59(42):18619-18626. doi: , 10.1002/anie.202005505. Epub 2020 Aug 17. PMID:32533629[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Simmons CR, MacCulloch T, Zhang F, Liu Y, Stephanopoulos N, Yan H. A Self-Assembled Rhombohedral DNA Crystal Scaffold with Tunable Cavity Sizes and High-Resolution Structural Detail. Angew Chem Int Ed Engl. 2020 Oct 12;59(42):18619-18626. PMID:32533629 doi:10.1002/anie.202005505
