7sku
From Proteopedia
Nipah virus matrix protein in complex with PI(4,5)P2
Structural highlights
FunctionMATRX_NIPAV Plays a crucial role in virion assembly and budding. Forms a shell at the inner face of the plasma membrane (PubMed:17005661, PubMed:17204159, PubMed:19000317). Transits through the host nucleus before gaining the functional ability to localize and bud from the plasma membrane (PubMed:21085610). Mediates together with fusion protein the incorporation of the glycoprotein to the viral particles (PubMed:28250132). Participates also in the inhibition of the host interferon type I antiviral response by interacting with and thereby inhibiting host TRIM6 (PubMed:27622505).[1] [2] [3] [4] [5] [6] Publication Abstract from PubMedMeasles virus, Nipah virus, and multiple other paramyxoviruses cause disease outbreaks in humans and animals worldwide. The paramyxovirus matrix (M) protein mediates virion assembly and budding from host cell membranes. M is thus a key target for antivirals, but few high-resolution structures of paramyxovirus M are available, and we lack the clear understanding of how viral M proteins interact with membrane lipids to mediate viral assembly and egress that is needed to guide antiviral design. Here, we reveal that M proteins associate with phosphatidylserine and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] at the plasma membrane. Using x-ray crystallography, electron microscopy, and molecular dynamics, we demonstrate that PI(4,5)P2 binding induces conformational and electrostatic changes in the M protein surface that trigger membrane deformation, matrix layer polymerization, and virion assembly. Measles and Nipah virus assembly: Specific lipid binding drives matrix polymerization.,Norris MJ, Husby ML, Kiosses WB, Yin J, Saxena R, Rennick LJ, Heiner A, Harkins SS, Pokhrel R, Schendel SL, Hastie KM, Landeras-Bueno S, Salie ZL, Lee B, Chapagain PP, Maisner A, Duprex WP, Stahelin RV, Saphire EO Sci Adv. 2022 Jul 22;8(29):eabn1440. doi: 10.1126/sciadv.abn1440. Epub 2022 Jul, 20. PMID:35857835[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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