Structural highlights
Function
FRSA_VIBVU Catalyzes the hydrolysis of esters (PubMed:30951551). In vitro, prefers short chain alkanoate ester as substrate. Displays highest activity towards p-nitrophenyl acetate (pNPA). Has weaker activity towards p-nitrophenyl butyrate (pNPB) (PubMed:30951551).[1]
Publication Abstract from PubMed
The interaction between fermentation-respiration switch (FrsA) protein and glucose-specific enzyme IIA(Glc) increases glucose fermentation under oxygen-limited conditions. We show that FrsA converts pyruvate to acetaldehyde and carbon dioxide in a cofactor-independent manner and that its pyruvate decarboxylation activity is enhanced by the dephosphorylated form of IIA(Glc) (d-IIA(Glc)). Crystal structures of FrsA and its complex with d-IIA(Glc) revealed residues required for catalysis as well as the structural basis for the activation by d-IIA(Glc).
FrsA functions as a cofactor-independent decarboxylase to control metabolic flux.,Lee KJ, Jeong CS, An YJ, Lee HJ, Park SJ, Seok YJ, Kim P, Lee JH, Lee KH, Cha SS Nat Chem Biol. 2011 May 29. PMID:21623357[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wang X, Li ZM, Li Q, Shi M, Bao L, Xu D, Li Z. Purification and biochemical characterization of FrsA protein from Vibrio vulnificus as an esterase. PLoS One. 2019 Apr 5;14(4):e0215084. PMID:30951551 doi:10.1371/journal.pone.0215084
- ↑ Lee KJ, Jeong CS, An YJ, Lee HJ, Park SJ, Seok YJ, Kim P, Lee JH, Lee KH, Cha SS. FrsA functions as a cofactor-independent decarboxylase to control metabolic flux. Nat Chem Biol. 2011 May 29. PMID:21623357 doi:10.1038/nchembio.589
