Structural highlights
8bty is a 2 chain structure with sequence from Streptococcus agalactiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.7Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
Q53637_STRAG This virulence factor of S.pyogenes specifically cleaves the human serum chemotaxin C5a at '68-Lys-|-Asp-69' bond near its C-terminus, destroying its ability to serve as a chemoattractant.[ARBA:ARBA00002909]
Publication Abstract from PubMed
A 1.7 A structure is presented for an active form of the virulence factor ScpB, the C5a peptidase from Streptococcus agalactiae. The previously reported structure of the ScpB active site mutant exhibited a large separation (~20 A) between the catalytic His and Ser residues. Significant differences are observed in the catalytic domain between the current and mutant ScpB structures resulting with a high RMSD(Calpha) (4.6 A). The fold of the active form of ScpB is nearly identical to ScpA (RMSD(Calpha) 0.2 A), the C5a-peptidase from Streptococcus pyogenes. Both ScpA and ScpB have comparable activity against human C5a, indicating neither enzyme require host proteins for C5a-ase activity. These studies are a first step in resolving reported differences in the specificities of these enzymes.
The 1.7 A crystal structure of the C5a peptidase from Streptococcus agalactiae (ScpB) reveals an active site competent for catalysis.,Cullen R, Tecza M, Miclot T, Behan S, Jain M, Avink MK, Cooney JC, Kagawa TF Proteins. 2023 Nov 3. doi: 10.1002/prot.26625. PMID:37921533[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cullen R, Teçza M, Miclot T, Behan S, Jain M, Avink MK, Cooney JC, Kagawa TF. The 1.7 Å crystal structure of the C5a peptidase from Streptococcus agalactiae (ScpB) reveals an active site competent for catalysis. Proteins. 2023 Nov 3. PMID:37921533 doi:10.1002/prot.26625
