1pbv
From Proteopedia
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SEC7 DOMAIN OF THE EXCHANGE FACTOR ARNO
Overview
Small G proteins switch from a resting, GDP-bound state to an active, GTP-bound state. As spontaneous GDP release is slow, guanine-nucleotide-exchange factors (GEFs) are required to promote fast, activation of small G proteins through replacement of GDP with GTP in, vivo. Families of GEFs with no sequence similarity to other GEF families, have now been assigned to most families of small G proteins. In the case, of the small G protein Arf1, the exchange of bound GDP for GTP promotes, the coating of secretory vesicles in Golgi traffic. An exchange factor for, human Arf1, ARNO, and two closely related proteins, named cytohesin 1 and, GPS1, have been identified. These three proteins are modular proteins with, an amino-terminal coiled-coil, a central Sec7-like domain and a, carboxy-terminal pleckstrin homology domain. The Sec7 domain contains the, exchange-factor activity. It was first found in Sec7, a yeast protein, involved in secretion, and is present in several other proteins, including, the yeast exchange factors for Arf, Geal and Gea2. Here we report the, crystal structure of the Sec7 domain of human ARNO at 2 A resolution and, the identification of the site of interaction of ARNO with Arf.
About this Structure
1PBV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the Sec7 domain of the Arf exchange factor ARNO., Cherfils J, Menetrey J, Mathieu M, Le Bras G, Robineau S, Beraud-Dufour S, Antonny B, Chardin P, Nature. 1998 Mar 5;392(6671):101-5. PMID:9510256
Page seeded by OCA on Mon Nov 12 18:41:56 2007
