5z8o

Publication Abstract from PubMed

Mycobacterium tuberculosis is a notorious pathogen that continues to threaten human health. Rv0164, an antigen of both T- and B-cells conserved across mycobacteria, and MSMEG_0129, its close homolog in Mycobacterium smegmatis, are predicted members of the START domain superfamily, but their molecular function is unknown. Here, gene knockout studies demonstrate MSMEG_0129 is essential for bacterial growth, suggesting Rv0164 may be a potential drug target. The MSMEG_0129 crystal structure determined at 1.95 A reveals a fold similar to that in polyketide aromatase/cyclases ZhuI and TcmN from Streptomyces sp. Structural comparisons and docking simulations, however, infer that MSMEG_0129 and Rv0164 are unlikely to catalyze polyketide aromatization/cyclization, but probably play an irreplaceable role during mycobacterial growth, for example in lipid transfer during cell envelope synthesis. This article is protected by copyright. All rights reserved.

Structural and genetic analysis of START superfamily protein MSMEG_0129 from Mycobacterium smegmatis.,Zheng S, Zhou Y, Fleming J, Zhou Y, Zhang M, Li S, Li H, Sun B, Liu W, Bi L FEBS Lett. 2018 Mar 7. doi: 10.1002/1873-3468.13024. PMID:29512898^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.