1ppg
From Proteopedia
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THE REFINED 2.3 ANGSTROMS CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE ELASTASE IN A COMPLEX WITH A VALINE CHLOROMETHYL KETONE INHIBITOR
Contents |
Overview
The stoichiometric complex formed between human leukocyte elastase and a, synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was, co-crystallized and its X-ray structure determined, using Patterson search, methods. Its structure has been crystallographically refined to a final R, value of 0.145 (8.0 and 2.3 A). The enzyme structure is very similar to, that recently observed in a complex formed with the ovomucoid third domain, from turkey [(1986) EMBO J. 5,2453-2458]. The rms deviation of all, alpha-carbon atoms is 0.32 A. The peptidic inhibitor is bound in a similar, overall conformation as the ovomucoid binding segment. Covalent bonds are, formed between Val-P1 of the inhibitor and His-57 NE2 and Ser-195 OG of, the enzyme. The carbonyl carbon is tetrahedrally deformed to a hemiketal., The valine side chain is arranged in the S1 pocket in the g-conformation.
Disease
Known diseases associated with this structure: Hematopoiesis, cyclic OMIM:[130130], Neutropenia, congenital OMIM:[130130]
About this Structure
1PPG is a Single protein structure of sequence from [1]. Active as Leukocyte elastase, with EC number 3.4.21.37 Full crystallographic information is available from OCA.
Reference
The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor., Wei AZ, Mayr I, Bode W, FEBS Lett. 1988 Jul 18;234(2):367-73. PMID:3391280
Page seeded by OCA on Mon Nov 12 18:46:06 2007
