Structural highlights
Function
AGCT1_HORVU Involved in the synthesis of hordatines (antifungal hydroxycinnamoylagmatine derivatives). Specific for agmatine as the acyl acceptor, inactive towards tyramine and putrescine. Has activity with the acyl donors 4-coumaroyl-CoA, cinnamoyl-CoA, caffeoyl-CoA, feruloyl-CoA, and to a lesser extent sinapoyl-CoA.[1] [2]
Publication Abstract from PubMed
The enzymes of the BAHD superfamily, a large group of acyl-CoA-dependent acyltransferases in plants, are involved in the biosynthesis of diverse secondary metabolites. While the structures of several O-acyltransferases from the BAHD superfamily, such as hydroxycinnamoyl-CoA shikimate hydroxycinnamoyl transferase, have been elucidated, no structural information on N-acyltransferases is available. Hordeum vulgare agmatine coumaroyltransferase (HvACT) is an N-acyltransferase from the BAHD superfamily and is one of the most important enzymes in the secondary metabolism of barley. Here, an apo-form structure of HvACT is reported as the first structure of an N-acyltransferase from the BAHD superfamily. HvACT crystals diffracted to 1.8 A resolution and belonged to the monoclinic space group P21, with unit-cell parameters a = 57.6, b = 59.5, c = 73.6 A, alpha = 90, beta = 91.3 , gamma = 90 degrees . Like other known BAHD superfamily structures, HvACT contains two domains that adopt a two-layer alphabeta-sandwich architecture and a solvent-exposed channel that penetrates the enzyme core.
Crystal structure of barley agmatine coumaroyltransferase, an N-acyltransferase from the BAHD superfamily.,Yamane M, Takenoya M, Yajima S, Sue M Acta Crystallogr F Struct Biol Commun. 2020 Dec 1;76(Pt 12):590-596. doi:, 10.1107/S2053230X20014880. Epub 2020 Nov 25. PMID:33263570[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Burhenne K, Kristensen BK, Rasmussen SK. A new class of N-hydroxycinnamoyltransferases. Purification, cloning, and expression of a barley agmatine coumaroyltransferase (EC 2.3.1.64). J Biol Chem. 2003 Apr 18;278(16):13919-27. PMID:12582168 doi:10.1074/jbc.M213041200
- ↑ Nomura T, Ishizuka A, Kishida K, Islam AK, Endo TR, Iwamura H, Ishihara A. Chromosome arm location of the genes for the biosynthesis of hordatines in barley. Genes Genet Syst. 2007 Dec;82(6):455-64. PMID:18270436 doi:10.1266/ggs.82.455
- ↑ Yamane M, Takenoya M, Yajima S, Sue M. Crystal structure of barley agmatine coumaroyltransferase, an N-acyltransferase from the BAHD superfamily. Acta Crystallogr F Struct Biol Commun. 2020 Dec 1;76(Pt 12):590-596. PMID:33263570 doi:10.1107/S2053230X20014880
