Structural highlights
Function
F8JK18_STREN
Publication Abstract from PubMed
The crystal structures of cattleyene synthase (apo-CyS), and CyS complexed with geranylgeranyl pyrophosphate (GGPP) were solved. The CyS(C59A) variant exhibited an increased production of cattleyene and other diterpenes with diverse skeletons. Its structure showed a widened active site cavity explaining the relaxed selectivity. Isotopic labeling experiments revealed a remarkable cyclization mechanism involving several skeletal rearrangements for one of the novel diterpenes.
Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes.,Xing B, Xu H, Li A, Lou T, Xu M, Wang K, Xu Z, Dickschat JS, Yang D, Ma M Angew Chem Int Ed Engl. 2022 Sep 5;61(36):e202209785. doi: , 10.1002/anie.202209785. Epub 2022 Aug 1. PMID:35819825[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Xing B, Xu H, Li A, Lou T, Xu M, Wang K, Xu Z, Dickschat JS, Yang D, Ma M. Crystal Structure Based Mutagenesis of Cattleyene Synthase Leads to the Generation of Rearranged Polycyclic Diterpenes. Angew Chem Int Ed Engl. 2022 Sep 5;61(36):e202209785. doi: , 10.1002/anie.202209785. Epub 2022 Aug 1. PMID:35819825 doi:http://dx.doi.org/10.1002/anie.202209785
