Structural highlights
Function
RS5_MYCS2 With S4 and S12 plays an important role in translational accuracy.[HAMAP-Rule:MF_01307] Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body.[HAMAP-Rule:MF_01307]
Publication Abstract from PubMed
The ribosome carries out the synthesis of proteins in every living cell. It consequently represents a frontline target in anti-microbial therapy. Tuberculosis ranks among the leading causes of death worldwide, due in large part to the combination of difficult-to-treat latency and antibiotic resistance. Here, we present the 3.3-A cryo-EM structure of the 70S ribosome of Mycobacterium smegmatis, a close relative to the human pathogen Mycobacterium tuberculosis. The structure reveals two additional ribosomal proteins and localizes them to the vicinity of drug-target sites in both the catalytic center and the decoding site of the ribosome. Furthermore, we visualized actinobacterium-specific rRNA and protein expansions that extensively remodel the ribosomal surface with implications for polysome organization. Our results provide a foundation for understanding the idiosyncrasies of mycobacterial translation and reveal atomic details of the structure that will facilitate the design of anti-tubercular therapeutics.
The Complete Structure of the Mycobacterium smegmatis 70S Ribosome.,Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N Cell Rep. 2017 Jul 5;20(1):149-160. doi: 10.1016/j.celrep.2017.06.029. PMID:28683309[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N. The Complete Structure of the Mycobacterium smegmatis 70S Ribosome. Cell Rep. 2017 Jul 5;20(1):149-160. doi: 10.1016/j.celrep.2017.06.029. PMID:28683309 doi:http://dx.doi.org/10.1016/j.celrep.2017.06.029
