Structural highlights
Function
CSC1_MOUSE Acts as an osmosensitive calcium-permeable cation channel (PubMed:27045885). Required for the functional integrity of the kidney glomerular filtration barrier (By similarity).[UniProtKB:D3ZNF5][1]
Publication Abstract from PubMed
The TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated in various physiological functions. Deficiency of these channels would cause many diseases including hearing loss. However, their structures and physiological roles are not yet well understood. In this study, we determine the cryo-electron microscopy (cryo-EM) structure of the mouse TMEM63C at 3.56 A, and revealed structural differences compared to TMEM63A, TMEM63B, and the plant orthologues OSCAs. Further structural guided mutagenesis and calcium imaging demonstrated the important roles of the coupling of TM0 and TM6 in channel activity. Additionally, we confirm that TMEM63C exists primarily as a monomer under physiological conditions, in contrast, TMEM63B is a mix of monomer and dimer in cells, suggesting that oligomerization is a regulatory mechanism for TMEM63 proteins.
Cryo-EM structure of TMEM63C suggests it functions as a monomer.,Qin Y, Yu D, Wu D, Dong J, Li WT, Ye C, Cheung KC, Zhang Y, Xu Y, Wang Y, Shi YS, Dang S Nat Commun. 2023 Nov 9;14(1):7265. doi: 10.1038/s41467-023-42956-2. PMID:37945568[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhao X, Yan X, Liu Y, Zhang P, Ni X. Co-expression of mouse TMEM63A, TMEM63B and TMEM63C confers hyperosmolarity activated ion currents in HEK293 cells. Cell Biochem Funct. 2016 Jun;34(4):238-41. PMID:27045885 doi:10.1002/cbf.3185
- ↑ Qin Y, Yu D, Wu D, Dong J, Li WT, Ye C, Cheung KC, Zhang Y, Xu Y, Wang Y, Shi YS, Dang S. Cryo-EM structure of TMEM63C suggests it functions as a monomer. Nat Commun. 2023 Nov 9;14(1):7265. PMID:37945568 doi:10.1038/s41467-023-42956-2
