1qbg
From Proteopedia
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CRYSTAL STRUCTURE OF HUMAN DT-DIAPHORASE (NAD(P)H OXIDOREDUCTASE)
Contents |
Overview
The crystal structure of human DT-diaphorase (NAD(P)H oxidoreductase, (quinone); EC 1.6.99.2) has been determined to 2.3 A resolution. There are, only minor differences in shape and volume between the active sites of the, rat and human enzymes and in the hydrophobic environment in the vicinity, of the substrate. The isoalloxazine ring of the bound FAD is more buried, in the human structure. Molecular modeling was used to examine optimal, positions for the antitumor prodrug CB1954, (5-(aziridin-1-yl)-2,4-dinitrobenzamide) in both the human and rat enzyme, active sites. This suggests that the position of CB1954 in the active site, of the human enzyme is very similar to that in the rat, although there are, detailed differences in the predicted patterns of hydrogen bonding between, side chains and the drug. Some of the differences are a consequence of the, shift in position for the FAD molecule and may contribute to the observed, differences in rate of the two-electron reduction of CB1954.
Disease
Known diseases associated with this structure: Benzene toxicity, susceptibility to OMIM:[125860], Leukemia, post-chemotherapy, susceptibility to OMIM:[125860]
About this Structure
1QBG is a Single protein structure of sequence from Homo sapiens with FAD as ligand. Active as NAD(P)H dehydrogenase (quinone), with EC number 1.6.5.2 Full crystallographic information is available from OCA.
Reference
Crystal structure of human DT-diaphorase: a model for interaction with the cytotoxic prodrug 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)., Skelly JV, Sanderson MR, Suter DA, Baumann U, Read MA, Gregory DS, Bennett M, Hobbs SM, Neidle S, J Med Chem. 1999 Oct 21;42(21):4325-30. PMID:10543876
Page seeded by OCA on Mon Nov 12 18:52:04 2007
