1qfw
From Proteopedia
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TERNARY COMPLEX OF HUMAN CHORIONIC GONADOTROPIN WITH FV ANTI ALPHA SUBUNIT AND FV ANTI BETA SUBUNIT
Overview
Human chorionic gonadotropin (hCG), is a placental hormone which exerts, its major effect by stimulating progesterone production, crucially, sustaining the early weeks of pregnancy. Detection of hCG with specific, monoclonal antibodies (mAbs) has become the chosen means for pregnancy, diagnosis. We have used antibody Fv fragments derived from two, high-affinity mAbs, one against the alpha and the other against the, beta-hCG subunit to enable the crystallisation of intact or desialylated, hCG. Crystals of a ternary complex composed of Fv anti-alpha/hCG/Fv, anti-beta were found to diffract to 3.5 A resolution, and the structure, was solved by molecular replacement. In the crystal, the two Fvs keep hCG, as in a molecular cage, providing good protein-protein contacts and, leaving enough space for the saccharides to be accommodated in the cell, solvent. The two Fvs were found not to interact directly through their, complementary-determining regions with the hCG saccharides, but only with, the protein. The hCG structure in the ternary complex was very close to, that of the HF partially deglycosylated hormone, thus indicating that, neither the saccharides nor the Fvs had any substantial influence on, hormone structure.
About this Structure
1QFW is a Protein complex structure of sequences from Homo sapiens and Mus musculus with NAG as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of a ternary complex between human chorionic gonadotropin (hCG) and two Fv fragments specific for the alpha and beta-subunits., Tegoni M, Spinelli S, Verhoeyen M, Davis P, Cambillau C, J Mol Biol. 1999 Jun 25;289(5):1375-85. PMID:10373373
Page seeded by OCA on Mon Nov 12 18:52:44 2007
