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3zqu

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Revision as of 11:10, 20 December 2023 by OCA (Talk | contribs)

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STRUCTURE OF A PROBABLE AROMATIC ACID DECARBOXYLASE

Structural highlights

3zqu is a 1 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.5Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

UBIX_PSEAE Flavin prenyltransferase that catalyzes the synthesis of the prenylated FMN cofactor (prenyl-FMN) for 4-hydroxy-3-polyprenylbenzoic acid decarboxylase UbiD. The prenyltransferase is metal-independent and links a dimethylallyl moiety from dimethylallyl monophosphate (DMAP) to the flavin N5 and C6 atoms of FMN.[HAMAP-Rule:MF_01984]^[1]

Publication Abstract from PubMed

The ubiX gene (PA4019) of Pseudomonas aeruginosa has been annotated as encoding a putative 3-octaprenyl-4-hydroxybenzoate decarboxylase from the ubiquinone-biosynthesis pathway. Based on a transposon mutagenesis screen, this gene was also implicated as being essential for the survival of this organism. The crystal structure of recombinant UbiX determined to 1.5 A resolution showed that the protein belongs to the superfamily of homo-oligomeric flavine-containing cysteine decarboxylases. The enzyme assembles into a dodecamer with 23 point symmetry. The subunit displays a typical Rossmann fold and contains one FMN molecule bound at the interface between two subunits.

Structure of PA4019, a putative aromatic acid decarboxylase from Pseudomonas aeruginosa.,Kopec J, Schnell R, Schneider G Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1184-8., Epub 2011 Sep 24. PMID:22102023^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ White MD, Payne KA, Fisher K, Marshall SA, Parker D, Rattray NJ, Trivedi DK, Goodacre R, Rigby SE, Scrutton NS, Hay S, Leys D. UbiX is a flavin prenyltransferase required for bacterial ubiquinone biosynthesis. Nature. 2015 Jun 25;522(7557):502-6. doi: 10.1038/nature14559. Epub 2015 Jun 17. PMID:26083743 doi:http://dx.doi.org/10.1038/nature14559
↑ Kopec J, Schnell R, Schneider G. Structure of PA4019, a putative aromatic acid decarboxylase from Pseudomonas aeruginosa. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1184-8., Epub 2011 Sep 24. PMID:22102023 doi:10.1107/S174430911102923X