1qu6
From Proteopedia
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STRUCTURE OF THE DOUBLE-STRANDED RNA-BINDING DOMAIN OF THE PROTEIN KINASE PKR REVEALS THE MOLECULAR BASIS OF ITS DSRNA-MEDIATED ACTIVATION
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Overview
Protein kinase PKR is an interferon-induced enzyme that plays a key role, in the control of viral infections and cellular homeostasis. Compared with, other known kinases, PKR is activated by a distinct mechanism that, involves double-stranded RNA (dsRNA) binding in its N-terminal region in, an RNA sequence-independent fashion. We report here the solution structure, of the 20 kDa dsRNA-binding domain (dsRBD) of human PKR, which provides, the first three-dimensional insight into the mechanism of its, dsRNA-mediated activation. The structure of dsRBD exhibits a dumb-bell, shape comprising two tandem linked dsRNA-binding motifs (dsRBMs) both with, an alpha-beta-beta-beta-alpha fold. The structure, combined with previous, mutational and biochemical data, reveals a highly conserved RNA-binding, site on each dsRBM and suggests a novel mode of protein-RNA recognition., The central linker is highly flexible, which may enable the two dsRBMs to, wrap around the RNA duplex for cooperative and high-affinity binding, leading to the overall change of PKR conformation and its activation.
Disease
Known disease associated with this structure: Kallmann syndrome 3 OMIM:[607123]
About this Structure
1QU6 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the double-stranded RNA-binding domain of the protein kinase PKR reveals the molecular basis of its dsRNA-mediated activation., Nanduri S, Carpick BW, Yang Y, Williams BR, Qin J, EMBO J. 1998 Sep 15;17(18):5458-65. PMID:9736623
Page seeded by OCA on Mon Nov 12 18:56:40 2007
