8reb
From Proteopedia
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 6nt complex
Structural highlights
FunctionRPOA_ECOLI DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit plays an important role in subunit assembly since its dimerization is the first step in the sequential assembly of subunits to form the holoenzyme.[HAMAP-Rule:MF_00059] Publication Abstract from PubMedGene transcription is a fundamental cellular process carried out by RNA polymerase (RNAP). Transcription initiation is highly regulated, and in bacteria, transcription initiation is mediated by sigma (sigma) factors. sigma recruits RNAP to the promoter DNA region, located upstream of the transcription start site (TSS) and facilitates open complex formation, where double-stranded DNA is opened up into a transcription bubble and template strand DNA is positioned inside RNAP for initial RNA synthesis. During initial transcription, RNAP remains bound to sigma and upstream DNA, presumably with an enlarging transcription bubble. The release of RNAP from upstream DNA is required for promoter escape and processive transcription elongation. Bacteria sigma factors can be broadly separated into two classes with the majority belonging to the sigma(70) class, represented by the sigma(70) that regulates housekeeping genes. sigma(54) forms a class on its own and regulates stress response genes. Extensive studies on sigma(70) have revealed the molecular mechanisms of the sigma(70) dependent process while how sigma(54) transitions from initial transcription to elongation is currently unknown. Here, we present a series of cryo-electron microscopy structures of the RNAP-sigma(54) initial transcribing complexes with progressively longer RNA, which reveal structural changes that lead to promoter escape. Our data show that initially, the transcription bubble enlarges, DNA strands scrunch, reducing the interactions between sigma(54) and DNA strands in the transcription bubble. RNA extension and further DNA scrunching help to release RNAP from sigma(54) and upstream DNA, enabling the transition to elongation. Structural basis of sigma(54) displacement and promoter escape in bacterial transcription.,Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X Proc Natl Acad Sci U S A. 2024 Jan 9;121(2):e2309670120. doi: , 10.1073/pnas.2309670120. Epub 2024 Jan 3. PMID:38170755[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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