8qxp

Publication Abstract from PubMed

Conjugation is the process by which plasmids, including those that carry antibiotic-resistance genes, are mobilized from one bacterium (the donor) to another (the recipient). The conjugation efficiency of IncF-like plasmids relies on the formation of mating-pair stabilization via intimate interactions between outer membrane proteins on the donor (a plasmid-encoded TraN isoform) and recipient bacteria. Conjugation of the R100-1 plasmid into Escherichia coli and Klebsiella pneumoniae (KP) recipients relies on pairing between the plasmid-encoded TraNalpha in the donor and OmpW in the recipient. Here, the crystal structure of K. pneumoniae OmpW (OmpW(KP)) is reported at 3.2 A resolution. OmpW(KP) forms an eight-stranded beta-barrel flanked by extracellular loops. The structures of E. coli OmpW (OmpW(EC)) and OmpW(KP) show high conservation despite sequence variability in the extracellular loops.

Structure of the outer membrane porin OmpW from the pervasive pathogen Klebsiella pneumoniae.,Seddon C, Frankel G, Beis K Acta Crystallogr F Struct Biol Commun. 2024 Jan 1;80(Pt 1):22-27. doi: , 10.1107/S2053230X23010579. Epub 2024 Jan 1. PMID:38206593^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.