Structural highlights
Function
DYR_ECOLI Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.
Publication Abstract from PubMed
We introduce an engineered nanobody whose affinity to green fluorescent protein (GFP) can be switched on and off with small molecules. By controlling the cellular localization of GFP fusion proteins, the engineered nanobody allows interrogation of their roles in basic biological processes, an approach that should be applicable to numerous previously described GFP fusions. We also outline how the binding affinities of other nanobodies can be controlled by small molecules.
Chemogenetic Control of Nanobodies.,Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi:, 10.1038/s41592-020-0746-7. PMID:32066961[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Farrants H, Tarnawski M, Muller TG, Otsuka S, Hiblot J, Koch B, Kueblbeck M, Krausslich HG, Ellenberg J, Johnsson K. Chemogenetic Control of Nanobodies. Nat Methods. 2020 Feb 17. pii: 10.1038/s41592-020-0746-7. doi:, 10.1038/s41592-020-0746-7. PMID:32066961 doi:http://dx.doi.org/10.1038/s41592-020-0746-7
