1rhg
From Proteopedia
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THE STRUCTURE OF GRANULOCYTE-COLONY-STIMULATING FACTOR AND ITS RELATIONSHIP TO THOSE OF OTHER GROWTH FACTORS
Overview
We have determined the three-dimensional structure of recombinant human, granulocyte-colony-stimulating factor by x-ray crystallography. Phases, were initially obtained at 3.0-A resolution by multiple isomorphous, replacement and were refined by solvent flattening and by averaging of the, electron density of the three molecules in the asymmetric unit. The, current R factor is 21.5% for all data between 6.0- and 2.2-A resolution., The structure is predominantly helical, with 104 of the 175 residues, forming a four-alpha-helix bundle. The only other secondary structure is, also helical. In the loop between the first two long helices a, four-residue 3(10)-helix is immediately followed by a 6-residue, alpha-helix. Three residues in the short connection between the second and, third bundle helices form almost one turn of left-handed helix. The, up-up-down-down connectivity with two long crossover connections has been, reported previously for five other proteins, which like, granulocyte-colony-stimulating factor are all signaling ligands: growth, hormone, granulocyte/macrophage-colony-stimulating factor, interferon, beta, interleukin 2, and interleukin 4. Structural similarity among these, growth factors occurs despite the absence of similarity in their amino, acid sequences. Conservation of this tertiary structure suggests that, these different growth factors might all bind to their respective, sequence-related receptors in an equivalent manner.
About this Structure
1RHG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors., Hill CP, Osslund TD, Eisenberg D, Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5167-71. PMID:7685117
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