Structural highlights
6tkv is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.95Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
FUT8_HUMAN Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.[1]
Publication Abstract from PubMed
Core-fucosylation is an essential biological modification by which a fucose is transferred from GDP-beta-L-fucose to the innermost N-acetylglucosamine residue of N-linked glycans. A single human enzyme alpha1,6-fucosyltransferase (FUT8) is the only enzyme responsible for this modification via the addition of an alpha-1,6-linked fucose to N-glycans. To date, the details of substrate recognition and catalysis by FUT8 remain unknown. Here, we report the crystal structure of FUT8 complexed with GDP and a biantennary complex N-glycan (G0), which provides insight into both substrate recognition and catalysis. FUT8 follows an SN2 mechanism and deploys a series of loops and an alpha-helix which all contribute in forming the binding site. An exosite, formed by one of these loops and an SH3 domain, is responsible for the recognition of branched sugars, making contacts specifically to the alpha1,3 arm GlcNAc, a feature required for catalysis. This information serves as a framework for inhibitor design, and helps to assess its potential as a therapeutic target.
Structural basis for substrate specificity and catalysis of alpha1,6-fucosyltransferase.,Garcia-Garcia A, Ceballos-Laita L, Serna S, Artschwager R, Reichardt NC, Corzana F, Hurtado-Guerrero R Nat Commun. 2020 Feb 20;11(1):973. doi: 10.1038/s41467-020-14794-z. PMID:32080177[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yanagidani S, Uozumi N, Ihara Y, Miyoshi E, Yamaguchi N, Taniguchi N. Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells. J Biochem. 1997 Mar;121(3):626-32. PMID:9133635
- ↑ Garcia-Garcia A, Ceballos-Laita L, Serna S, Artschwager R, Reichardt NC, Corzana F, Hurtado-Guerrero R. Structural basis for substrate specificity and catalysis of alpha1,6-fucosyltransferase. Nat Commun. 2020 Feb 20;11(1):973. doi: 10.1038/s41467-020-14794-z. PMID:32080177 doi:http://dx.doi.org/10.1038/s41467-020-14794-z
