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Publication Abstract from PubMed

The family of NAD(P)H-dependent short-chain dehydrogenases/reductases (SDRs) comprises numerous biocatalysts capable of C=O or C=C reduction. The highly homologous noroxomaritidine reductase (NR) from Narcissus sp. aff. pseudonarcissus and Zt_SDR from Zephyranthes treatiae, however, are SDRs with an extended imine substrate scope. Comparison with a similar SDR from Asparagus officinalis (Ao_SDR) exhibiting keto-reducing activity, yet negligible imine-reducing capability, and mining the Short-Chain Dehydrogenase/Reductase Engineering Database indicated that NR and Zt_SDR possess a unique active-site composition among SDRs. Adapting the active site of Ao_SDR accordingly improved its imine-reducing capability. By applying the same strategy, an unrelated SDR from Methylobacterium sp. 77 (M77_SDR) with distinct keto-reducing activity was engineered into a promiscuous enzyme with imine-reducing activity, thereby confirming that the ability to reduce imines can be rationally introduced into members of the "classical" SDR enzyme family. Thus, members of the SDR family could be a promising starting point for protein approaches to generate new imine-reducing enzymes.

Crossing the Border: From Keto- to Imine Reduction in Short-Chain Dehydrogenases/Reductases.,Roth S, Stockinger P, Steff J, Steimle S, Sautner V, Tittmann K, Pleiss J, Muller M Chembiochem. 2020 Sep 14;21(18):2615-2619. doi: 10.1002/cbic.202000233. Epub 2020, Jul 2. PMID:32315494^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.