Structural highlights
Function
CNRR_CUPMC CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.[1] [2]
Publication Abstract from PubMed
CnrX is the metal sensor and signal modulator of the three-protein transmembrane signal transduction complex CnrYXH of Cupriavidus metallidurans CH34 that is involved in the setup of cnr (cobalt and nickel resistance). We have determined the atomic structure of the soluble domain of CnrX in its Ni-bound, Co-bound, or Zn-bound form. Ni and Co ions elicit a biological response, while the Zn-bound form is inactive. The structures presented here reveal the topology of intraprotomer and interprotomer interactions and the ability of metal-binding sites to fine-tune the packing of CnrX dimer as a function of the bound metal. These data suggest an allosteric mechanism to explain how the complex is switched on and how the signal is modulated by Ni or Co binding. These results provide clues to propose a model for signal propagation through the membrane in the complex.
Structural Basis for Metal Sensing by CnrX.,Trepreau J, Girard E, Maillard AP, de Rosny E, Petit-Haertlein I, Kahn R, Coves J J Mol Biol. 2011 Mar 21. PMID:21414325[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Grass G, Grosse C, Nies DH. Regulation of the cnr cobalt and nickel resistance determinant from Ralstonia sp. strain CH34. J Bacteriol. 2000 Mar;182(5):1390-8. PMID:10671463
- ↑ Tibazarwa C, Wuertz S, Mergeay M, Wyns L, van Der Lelie D. Regulation of the cnr cobalt and nickel resistance determinant of Ralstonia eutropha (Alcaligenes eutrophus) CH34. J Bacteriol. 2000 Mar;182(5):1399-409. PMID:10671464
- ↑ Trepreau J, Girard E, Maillard AP, de Rosny E, Petit-Haertlein I, Kahn R, Coves J. Structural Basis for Metal Sensing by CnrX. J Mol Biol. 2011 Mar 21. PMID:21414325 doi:10.1016/j.jmb.2011.03.014
